2026-03-12T04:24:23Z https://jin-ai.repo.nii.ac.jp/oai

oai:jin-ai.repo.nii.ac.jp:00000684 2023-06-20T16:20:07Z 9:34

SYPRO〓 Ruby を用いたタンパク質染色法の湯浴加熱による迅速・高感度化 Increased Speed and Performance of Protein Staining with SYPRO〓 Ruby Protein Gel Stain using Hot Water Incubation 尼子, 克己 アマコ, カツミ Amako, Katsumi 蛍光染色 プロテオミクス 湯浴 The author presents a novel rapid, low-cost staining protocol (hot water protocol) for proteins in a polyacrylamide gel using the fluorescent dye SYPRO〓 Ruby. Compared to the supplierrecommended protocol, the hot water protocol showed equivalent dynamic range and linearity, and a lower detection limit. Peptides prepared with in-gel digestion extracted from polyacrylamide gels stained using the hot water protocol showed a sequence coverage ratio equivalent to that obtained using the supplier-recommended protocol, and de novo sequencing could also be carried out without issue. In addition, use of the hot water protocol dramatically decreased speckling noises owing to prolonged staining time or storage of the dye solution. These results demonstrate the superiority of the hot water protocol over the supplier-recommended protocol. 仁愛大学 2017-03-31 jpn departmental bulletin paper VoR https://doi.org/10.57426/00000678 https://jin-ai.repo.nii.ac.jp/records/684 10.57426/00000678 21853363 仁愛大学研究紀要. 人間生活学部篇 Research journal of Jin-ai University, faculty of human life studies 8 35 40 https://jin-ai.repo.nii.ac.jp/record/684/files/BD00010282_001.pdf application/pdf 636.9 kB 2022-10-06