{"created":"2023-06-20T15:54:12.505704+00:00","id":684,"links":{},"metadata":{"_buckets":{"deposit":"6f15bd52-4886-465c-bc4f-a141b99203cc"},"_deposit":{"created_by":2,"id":"684","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"684"},"status":"published"},"_oai":{"id":"oai:jin-ai.repo.nii.ac.jp:00000684","sets":["9:34"]},"author_link":["2680","1654"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"40","bibliographicPageStart":"35","bibliographic_titles":[{"bibliographic_title":"仁愛大学研究紀要. 人間生活学部篇"},{"bibliographic_title":"Research journal of Jin-ai University, faculty of human life studies","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The author presents a novel rapid, low-cost staining protocol (hot water protocol) for proteins in a polyacrylamide gel using the fluorescent dye SYPRO〓 Ruby. Compared to the supplierrecommended protocol, the hot water protocol showed equivalent dynamic range and linearity, and a lower detection limit. Peptides prepared with in-gel digestion extracted from polyacrylamide gels stained using the hot water protocol showed a sequence coverage ratio equivalent to that obtained using the supplier-recommended protocol, and de novo sequencing could also be carried out without issue. In addition, use of the hot water protocol dramatically decreased speckling noises owing to prolonged staining time or storage of the dye solution. These results demonstrate the superiority of the hot water protocol over the supplier-recommended protocol.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.57426/00000678","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"仁愛大学"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21853363","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"尼子, 克己"},{"creatorName":"アマコ, カツミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1654","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Amako, Katsumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2680","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-10-06"}],"displaytype":"detail","filename":"BD00010282_001.pdf","filesize":[{"value":"636.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BD00010282_001.pdf","url":"https://jin-ai.repo.nii.ac.jp/record/684/files/BD00010282_001.pdf"},"version_id":"d9cbe312-9ae5-4117-a77b-614dd4d7a9df"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"蛍光染色","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテオミクス","subitem_subject_scheme":"Other"},{"subitem_subject":"湯浴","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"SYPRO〓 Ruby を用いたタンパク質染色法の湯浴加熱による迅速・高感度化","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"SYPRO〓 Ruby を用いたタンパク質染色法の湯浴加熱による迅速・高感度化"},{"subitem_title":"Increased Speed and Performance of Protein Staining with SYPRO〓 Ruby Protein Gel Stain using Hot Water Incubation","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"2","path":["34"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-04-12"},"publish_date":"2017-04-12","publish_status":"0","recid":"684","relation_version_is_last":true,"title":["SYPRO〓 Ruby を用いたタンパク質染色法の湯浴加熱による迅速・高感度化"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-06-20T16:20:07.855395+00:00"}